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Subramanian, R. B.
- optimization of Fusaric Acid Production by Fusarium oxysporum f Sp. Lycopersici Using Response Surface Methodology
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Authors
Affiliations
1 BRD School of Biosciences, Sardar Patel Univ., P.Box: 39, VV Nagar-388120, Gujarat, IN
1 BRD School of Biosciences, Sardar Patel Univ., P.Box: 39, VV Nagar-388120, Gujarat, IN
Source
Indian Journal of Science and Technology, Vol 3, No 4 (2010), Pagination: 411-416Abstract
Production of fusaric acid was maximized by employing response surface methodology with two level factorial design involving potato infusion (X1) and sucrose (X2) as variables, after optimizing the carbon and nitrogen source. The pvalue for each factor was <0.05 suggesting that these factors have significant effect on the production of fusaric acid. The optimized medium consisted 100 g/l potato infusion, 10 g/l sucrose for the production of 12.8 mg/l fusaric acid. Besides, different process parameters like pH and inoculums size were also standardized. Optimum pH and inoculums size were found to be 6.5 and 6 mm agar plug respectively. This is the first report of using RSM (Response Surface Methodology) for optimizing a medium for FA production. This technique can be used in increasing FA production at industrial scale for commercial product without augmentation of costly additives.Keywords
Fusaric Acid, Fusarium oxysporum f Sp. lycopersici, Response Surface MethodologyFull Text
References
- Abbas HK, Boyette CD, Hoagland RE and Vesonder RF (1991) Bioherbicidal potential of Fusarium moniliforme and its phytotoxin, fumonisin. Weed Sci. 39, 673–677.
- Amalfitano C, Pengue R, Andolfi A, Vurro M, Zonno MC and Evidente A (2002) HPLC analysis of FA, 9, 10-dehydrofusaric acid, their methyl esters, toxic metabolites from weed pathogenic Fusarium species. Phytochem. Analysis. 13, 277–282.
- Bacon CW, Porter JK, Norred WP and Leslie JF (1996) Production of fusaric acid by Fusarium species. Appl. Environ. Microbiol. 63, 4039–4043.
- Barna B, Sarhan ART and Kirtly Z (1983) The influence of nitrogen nutrition on the sensitivity of tomato plants to culture filtrates of Fusarium and to fusaric acid. Physiol. Plant Pathol. 23, 257-263.
- Box GEP and Wilson KB (1951) On the Experimental Attainment of Optimum Conditions. J. R. Stat. Soc. B. (13), 1-45.
- Capasso R, Evidente A, Cutignano A, Vurro M, Zonno MC and Bottalico A (1996) Fusaric and dehydrofusaric acids and their methyl esters from Fusarium nygamai. Phytochem. 41, 1035–1039.
- Claydon N, Grove JF and Pople M (1977) Fusaric acid from Fusarium solani. Phytochem. 16, 603.
- Duarte MLR and Archer SA (2003) In vitro toxin production by Fusarium solani f. sp. piperis. Fitopatologia Brasileira. 28, 229-235.
- Gäumann E (1957) Fusaric acid as a wilt toxin. Phytopathol. 47, 342–357.
- Haalland PD (1989) Experimental design in biotechnology, Marcel Dekker Inc, New York.
- Hidaka H, Nagatsu T and Takeya K (1969) Fusaric acid, a hypotensive agent produced by fungi. J. Antibiot. 22, 228–230.
- Hidaka H (1971) Fusaric (5-butylpicolinic) acid, an inhibitor of dopamine beta-hydroxylase, affects serotonin and noradrenaline. Nature. 231, 54-55.
- Ibrahim HM, Yusoff WMW, Hamid AA, Illias RM, Hassan O and Omar (2005) Optimization of medium for the production of β-cyclodextrin glucanotransferase using Central Composite Design (CCD). Proc. Biochem. 40, 753-758.
- Idris AE, Abouzeid MA, Boari A, Vurro M and Evidente A (2003) Identification of phytotoxic metabolites of a new Fusarium sp. inhibiting germination of Striga hermonthica seeds. Phytopathologia Mediterranea. 42, 65–70.
- Kern H (1972) Phytotoxins produced by Fusaria. In: Wood, R.K.S., Balili, A. & Graniti, A (Eds.) Phytotoxins Ppant Disease. Academic Press, New York. pp35-48.
- Khuri AI and Cornell JA (1987) Response surfaces: design and analysis. Marcel Dekker Inc., New York.
- Lee SL and Chen WC (1997) Optimization of medium composition for the production of glucosyltransferase by Aspergillus niger with response surface methodology. Enz. Microbial. Technol. 21, 436-440.
- Liu GQ and Wang XL (2007) Optimization of critical medium components using response surface methodology for biomass and extracellular polysaccharide production by Agaricus blazei. Appl. Microbiol. Biotechnol. 74, 78–83.
- Maddon IS and Richard SH (1997) Use of response surface methodology for rapid optimization of microbiological media. J. Appl. Bacteriol. 43, 197-204.
- Matta RJ and Wooten GF (1973) Pharmacology of fusaric acid in man. Clin. Pharmacol. Ther. 14(4), 541–546.
- Myers RH and Montgomery DC (1995) Response surface methodology: process and product optimization using designed experiments. 2nd ed. Wiley, New York.
- Nachmias S, Buchner V, Tsror L, Burstein Y and Keen N (1987) Differential phytotoxicity of peptides from culture fluids of Verticillium dahliae races 1 and 2 and their relationship to pathogenicity of the fungi on tomato. Phytopathol. 77, 506-510.
- Onesirosan P, Mabuni CT, Durbin RD, Morin RB, Rich DH and Arny DC (1975) Toxin production by Corynespora cassiicola. Physiol. Plant Pathol. 5, 298-295.
- Patel JD, Patil MR and Sapkal PN (1987) Toxic effect of culture filtrate of Curvularia lunata (Wakker) Boedijin and Alternaria tenuis Auct. PKV Res. J. 11,189.
- Pozuelo J (1976) Suppression of craving and withdrawal in humans addicted to narcotics or amphetamines by administration of alpha-methylpara- tvrosine (AMPT) and 5-butylpicolinic acid (fusaric acid). Cleve. Clin. Q. 43, 89-94.
- Rahman RA, Illias RM, Nawawi MGM, Ismail AF, Hassan O and Kamaruddin K (2004) Optimisation of growth medium for the production of cyclodextrin glucanotransferase from Bacillus stearothermophilus HR1 using response surface methodology. Proc. Biochem. 39, 2053-2060.
- Rudolf K (1976) Non-specific toxins. In: Heitefuss R. & Williams PH. (Eds.) Encyclopedia of Plant Physiology, New Series. Physiological Plant Pathology. Springer- Verlag. Berlin. 4, 279- 315.
- Stefan E (2005) Thin-layer chromatography-an appropriate method for fusaric acid estimation. Biologia Bratislava. 60(1), 104.
- Sulaiman SA, Hamid AA and Yusoff WMW (2005) Medium optimization for the production of biomass by Cunninghamella sp. 2a1 using response surface methodology. A.J.S.T.D. 22, 223-231.
- Zonno MC, Vurro M, Evidente A, Capasso R, Cutignano A and Sauerborn J (1996) Phytotoxic metabolites produced by Fusarium nygamai from Striga hermonthica. In Proc. IX Int. Symp. onbiological control of weeds. Stellenbosch, South Africa. pp223–226.
- A Method for Small Scale Genomic DNA Isolation from Chickpea (Cicer arietinum L.) Suitable for Molecular Marker Analysis
Abstract Views :558 |
PDF Views:121
Authors
Affiliations
1 B. R. Doshi School of Biosciences, Sardar Patel Maidan, Vadtal Road, Post Box No. 39, Sardar Patel University, Vallabh Vidyanagar-388120, Gujarat, IN
1 B. R. Doshi School of Biosciences, Sardar Patel Maidan, Vadtal Road, Post Box No. 39, Sardar Patel University, Vallabh Vidyanagar-388120, Gujarat, IN
Source
Indian Journal of Science and Technology, Vol 3, No 12 (2010), Pagination: 1214-1217Abstract
We describe a DNA isolation procedure for chickpea (Cicer arietinum L.) which is rapid and less expensive without involving ultra centrifugation or column purification steps. DNA preparation obtained from the present study was essentially suitable for PCR analysis which is one of the key steps in crop improvement programme through marker development and genetic engineering techniques. The yield of DNA ranged from 0.595- 5.550 μg/ml and the purity ratio was between 1.025- 2.010 indicating minimum levels of contaminating metabolites. The present protocol offers as a reliable, and consistent DNA isolation method for chickpea that yields large amount of pure&intact DNA.Keywords
DNA Isolation, Cicer arietinum, PCR AnalysisFull Text
References
- Do N and Adams RP (1991) A simple technique of removing plant polysaccharides contaminants from DNA. Biotechniques. 10, 162-166.
- Doyle JJ and Doyle JL (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem. Bull. 19, 11-15.
- Doyle JJ and Doyle JL (1990) Isolation of plant DNA from fresh tissue. Focus. 12, 13-15.
- Fang G, Hammer S and Groumet R (1992) A quick and inexpensive method for removing polysaccharides from plant genomic DNA. Biofeedback. 13, 52-54.
- Lodhi MA, Ye GN and Weeden NF (1994) A simple and efficient method for DNA extraction from grapevine cultivars and Vitis species. Plant Mol. Biol. Rep. 12, 6-13.
- Loomis MD (1974) Overcoming problems of phenolics and quinones in the isolation of plant enzymes and organelles. Methods Enzymol. 31, 528–544.
- Mace ES, Buhariwalla HK and Crouch JH (2003) A high-throughput DNA extraction protocol for tropical molecular breeding programs. Plant Mol. Biol. Rep. 21, 459a-459h.
- Merlo DJ and Kemp JD (1976) Effect of polysaccharides on kinetics of DNA. Plant Physiol. 58, 1522-1526.
- Pandey RN, Adams RP and Flournoy LE (1996) Inhibitions of random amplified poly-morphic DNAs (RAPDs) by plant polysaccharides. Plant Mol. Biol. Rep. 14, 17-22.
- Paterson AH, Brubaker CL and Wendel JF (1993) A rapid method for extraction of cotton (Gossypium spp.) genomic DNA suitable for RFLP and PCR analysis. Plant Mol. Biol. Rep. 11, 122-127.
- Pikart MJ and Villeponteau B (1993) Suppression of PCR amplification by high levels of RNA. Biotech.14, 24-25.
- Porebski S, Bailey LG and Baum BR (1997) Modification of a CTAB DNA extraction protocol for plants containing high polysaccharide and polyphenol components. Plant Mol. Biol. Rep. 15, 8–15.
- Sambrook J, Fritch EF and Maniatis T (1989) Molecular cloning: A laboratory manual. Cold Springer Harbor Laboratory Press, Cold Spring Harbor, NY.
- Scott KD and Playford J (1996) DNA extraction technique for PCR in rain forest plant species. Biotech. 20, 974-978.
- Sharma AD, Gill PK and Singh P (2002) DNA isolation from dry and fresh samples of polysaccharide rich plants. Plant Mol. Biol. Rep. 20, 415a– 415f.
- Sharma KK, Lavanya M and Anjaiah V (2000) A method for isolation and purification of peanut genomic DNA suitable for analytical applications. Plant Mol. Biol. Rep. 18, 393a-393h.
- Varshney RK, Horres R, Molina C, Nayak S, Jungmann R, Swamy P, Winter P, Jayashree B, Kahl G and Hoisington DA (2007) Extending the repertoire of microsatellite markers for genetic linkage mapping and germplasm screening in chickpea. J. SAT Agri. Res. 5(1), 1-3.
- Weishing K, Nybom H, Wolff K and Meyer W (1995) DNA isolation and purification. In: DNA fingerprinting in plants and fungi, CRC Press, Boca Raton, Florida, pp: 44-59.